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Image Search Results
Journal: Brain : a journal of neurology
Article Title: Connexin43 mimetic peptide reduces vascular leak and retinal ganglion cell death following retinal ischaemia.
doi: 10.1093/brain/awr338
Figure Lengend Snippet: Figure 3 Three hours of hypoxia and 6 h of reperfusion lead to significant endothelial cell death in vitro in rat brain microvascular endothelial cells (RBMVEC; A). Non-specific gap junction blocker carbenoxolone, non-specific hemichannel blocker LaCl3, and connexin43 (Cx43) mimetic peptide protected endothelial cells against hypoxic injury, with the number of viable cells significantly higher than no treatment, while scrambled peptide did not have any protective effects. The number of viable cells was expressed as percentage of the control without hypoxia. Hypoxia and reperfusion also lead to significant propidium iodide dye uptake into primary rat brain microvascular endothelial cells indicating open hemichannels (B). Carbenoxolone, LaCl3 and connexin43 mimetic peptide significantly prevented dye uptake compared to no treatment, indicating hemichannel closure, while scrambled peptide did not have any effect. Stars denote statistical significance when compared to the control group or compared between groups in brackets; P 5 0.05.
Article Snippet: Rat brain microvascular endothelial cells (R840K-05a, Cell Applications) were plated into 24-well plates (1 105 cells/well) in
Techniques: In Vitro, Control
Journal: Journal of pharmacological and toxicological methods
Article Title: Simulated Traumatic Brain Injury in In-Vitro Mouse Neuronal and Brain Endothelial Cell Culture Models
doi: 10.1016/j.vascn.2022.107159
Figure Lengend Snippet: List of Used Material
Article Snippet: Mouse Neuronal Cell Culture Celprogen 11003-02 One primary brain cell line we used in the protocol Mouse Neuronal Cell Culture Complete Growth Glucose and Serum Free Media Celprogen M11003-02GF Flushed with hypoxic gas for hypoxic
Techniques: Sparging, Cell Culture, Sterility, Cell Counting, Proliferation Assay, End Point Assay, Activity Assay, CyQUANT Assay, Transferring, Light Microscopy, Saline, LDH Cytotoxicity Assay, Membrane